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Nanoscale magnetic imaging or ferritin in a single cell



 Nanoscale magnetic imaging of ferritin in a single cell
LEFT – Experimental setup. The experiment was carried out on a homebuilt setup, which combined optically detected magnetic resonance microscopy (ODMR) with atomic force microscopy (AFM). DM: dichroic mirror. BP: bandpass filter working at 650–775 nm. APD: avalanche photodiode. CCD: charge coupled device. LED: light emitting diode or 470 nm. AL: achromatic lens. PH: pinhole at a size of 30 μm. BS: beam splitter. RIGHT – Images of the nanopillars on diamonds. (A) SEM imaging of the fabricated diamond nanopillars just after reactive ion etching (RIE). The top of the nanopillar is covered by the hydrogen silsesquioxane (HSQ) to protect the NV center. (B) A single trapezoidal-cylinder shaped nanopillar to sense cell sections adhered to the AFM tip. Scale bars, 10 μm (A); 400 nm (B). Credit: Science Advances, doi: 10.1126 / sciadv.aau8038.
            

In life sciences, the ability to measure the distribution of biomolecules inside a cell in situ is an important investigative goal. Among other techniques, scientists have used magnetic imaging (MI) based on the nitrogen vacancy center (NV) in diamonds as a powerful tool in biomolecular research. However, nanoscale imaging of intracellular proteins has remained a challenge thus far. In a recent study now published in Science Advances Pengfei Wang and colleagues at the interdisciplinary departments of physics, biomacromolecules, quantum information and life sciences in China, used ferritin proteins to demonstrate the MI realization of endogenous proteins in a single cell, using the nitrogen-vacancy (NV) center as the sensor. They imaged intracellular ferritins and ferritin-containing organelles using MI and correlative electron microscopy for the nanoscale magnetic imaging (MI) or intracellular proteins.
                                               


Increasing existing spatial resolution of biomedical imaging is required to achieve ongoing demands in medical imaging, and therefore, among a variety of techniques, magnetic imaging or broad interest at present. Magnetic resonance imaging (MRI) is widely used to quantify the distribution of nuclear spins but conventional MRI can only reach a resolution of 1 µm in nuclear spin imaging where the resolution is limited by electrical detection sensitivity. Scientists have developed a series of techniques to break this resolution barrier, including a superconducting quantum interference device and magnetic resonance force microscopy. Nevertheless, these reports require a cryogenic environment and high vacuum for imaging, limiting the experimental implementation and its translation to clinical practice.

A recently developed quantum sensing method based on the nitrogen vacancy center in diamond has radically pushed the boundary of MI techniques to the nanoscale to detect organic molecules and proteins in the lab. Scientists have combined quantum sensing with NV centers and scanning probe microscopy to demonstrate nanoscale MRI for single electron spin and small nuclear spin ensemble while using the NV center as a biocompatible magnetometer for noninvasively image ferromagnetic particles within cells at the subcellular scale (0.4 µm). For example, depolarization of the NV center can be used as a wideband magnetometer to detect and measure fluctuating noise from metal ions and nuclear spins. However, such imaging of single proteins via MI at the nanoscale has not been reported in the single cell thus far.

 Nanoscale magnetic imaging of ferritin in a single cell
Schematic of the setup and experimental principle. (A) Schematic view of the experimental setup. The cell embedded in resin is attached to a tuning fork and scans above the diamond nanopillar that contains a shallow NV center. A copper wire is used to deliver the microwave pulse to the NV center. A green laser (532 nm) from the confocal microscope (CFM) is used to address, initialize, and read out the NV center. (B) Left: Crystal lattice and energy level of the NV center. The NV center is a defect that consists of a substitute nitrogen atom and an adjacent vacancy in diamond. Right: Schematic view of a ferritin. The black arrows indicate the electron spins or Fe3 +. (C) Experimental demonstration of the spin noise detection with and without ferritin in the form of polarization decay for the same NV center. The input is the pulse sequence for detection and imaging of the ferritin. A 5 μs green laser is used to initialize the spin state to ms = 0, followed by a free evolution time τ to accumulate the magnetic noise, and finally the spin state is read out by detecting the fluorescence intensity. The pulse sequence is repeated about 105 times to acquire a good signal-to-noise ratio (SNR). The relaxation time is adapted to be 0.1 and 3.3 ms by exponential decay for the case with and without ferritin, respectively, indicating a spin noise or 0.01 mT2. Credit: Science Advances, doi: 10.1126 / sciadv.aau8038.
            

In the present work, Wang et al. reported two technical advancements to allow nanoscale MI or intracellular proteins within a single cell. For this, they free-fixed the cell to a solid state and intricately segmented to a cube shape, then placed it on a tuning fork scanning probe or an atomic force microscope (AFM) for imaging, where the flat cross section of the cell was exposed to air. The scientists used the sample placement setup to allow the NV sensor to be located within 10 nm of the target proteins and used the AFM to suppress thermal drift during sample positioning. They then engineered trapezoidal cylinder-shaped nanopillars at a bulk diamond surface for image acquisition, technically shortening the time of image acquisition by one order compared to previous methods. In the present study, the scientists used this technique to conduct in situ MI of the magnetic fluctuating noise of intracellular ferritin proteins (a biomarker of iron stores and transferrin saturation in the body) within the experimental setup.

Ferritin is a globular protein complex with an outer diameter of 12 nm, containing a 8 nm cavity tension that allows up to 4500 iron atoms to be stored within the protein. The magnetic noise of ferric ions can be detected by their effects on the relaxation [of time] of an NV center. In this work, Wang et al. confirmed the observation using fluorescence measurements of time-dependent decay of the population of NV centers (magnetic spin, m S = 0 state), in a diamond surface coated with ferritins. Additionally, the scientists detected the magnetic noise with label-free methods using the NV center via transmission electron microscopy (TEM). The work allowed the development of a correlated MI and TEM to obtain and verify the first nanoscale MI of a protein in situ.

The scientists used the hepatic carcinoma cell line (HepG2) for the experiments and studied iron metabolism by treating the cells with ferric ammonium citrate (FAC), which significantly increased the amount of intracellular ferritin. They verified this using confocal microscopy (CFM), western blotting and TEM techniques at first. The results showed the primary localization of ferritins in the intracellular puncta around the nucleus, among the cytoplasm. The scientists used bulk electron paramagnetic resonance (EPR) spectroscopy to confirm the paramagnetic properties of ferritin in the FAC-treated HepG2 cells and mass spectroscopy to the interference due to other paramagnetic metal ions.

 Nanoscale magnetic imaging of ferritin in a single cell
TOP – The preparation and characterization of ferritin-rich HepG2 cell samples. (A) Schematic view of the treatment to cultured cells. Following iron loading or no treatment, the HepG2 cells were examined for fluorescence images and EPR spectra, respectively. For the MI and TEM imaging, cell samples were treated through high-pressure freezing, freeze substitution, and sectioning. (B) Representative confocal microscopy (CFM) image of ferritin structures (green) in iron-loaded HepG2 cells. The ferritin proteins were immunostained by anti-ferritin light chain antibody. The nuclei are indicated by 4 6, 6-diamidino-2-phenylindole (DAPI) in the blue channel. Inset displays magnified ferritin structures. The yellow dashed line outlines the contour of a cell. Scale bar, 20 μm. (C) EPR spectra of control and iron-loaded HepG2 cells at T = 300 K. BOTTOM – Adjusting the distance between the NV center and the cell section. (A) Interference fringes between the cell cube and the diamond surface. Scale bar, 20 μm. (B) The geometric relation and the gap R between cell samples and diamond pills for MI. The top surface diameter of the nanopillar is 400 nm. Credit: Science Advances, doi: 10.1126 / sciadv.aau8038.
            Wang et al. then used ultra fast, high pressure freezing to immobilize all intracellular components of the Fe loaded cells. The process stabilized the intracellular structures and molecules by minimizing Brownian motion in cells, which typically contributes to random motion of proteins up to 100 nm in vivo. To image the samples, they embedded and polymerized the frozen cells in LR White medium, followed by gluing the embedded cell sample to the AFM tuning for a few cells at the tip. Using a diamond knife, the scientists then sectioned the tip to nanometer flatness to examine the cuboid cell section under the AFM. They acquired MI images of ferritins by scanning the cellular cube along the diamond nanopillars and simultaneously measured NV spin repolarization rate using the "leapfrog" scanning mode of the microscope as detailed previously.

 Nanoscale magnetic imaging of ferritin in a single cell
Correlative MI and TEM images. (A) Schematic view of sectioning for correlative MI and TEM imaging. The last section and the remaining cube were transferred for TEM imaging and MI scanning, respectively. The sectioning resulted in some split ferritin clusters that could be imaged under both microscopes. A transparent blue strip of ~ 10 nm indicates the imaging depth of the MI, while in the TEM, the imaging depth is ~ 100 nm. (B) Distribution of ferritins from the last ultrathin section under TEM. Inset: Magnified figure of the part in a black dashed box. (C) MI result of the remaining cell cube. The pixel size is 43 nm. (D) The merged MI and TEM micrograph shows ferritins in a membrane-bound organelle. The red arrows in (B) to (D) indicate the same ferritin cluster. Scale bars, 5 μm (B) and 1 μm [B (inset), C, and D]. Credit: Science Advances, doi: 10.1126 / sciadv.aau8038.
            

The scientists measured fluorescence decay at a fixed free evolution time of 50 microseconds (τ = 50 μs) to reveal the degree of NV sensor spin polarization, which correlated with the amount of ferritin in the sensing volume. They observed the appearance of some clusters via both TEM and MI images, although some details were not observed in MI, the results confirmed that noise from intracellular ferritin contributed to depolarize the NV center. In order to obtain details of the ferritin clusters at higher resolution, the scientists minimized the pixel size to 8.3 nm and acquired MI of high resolution of the proteins as expected.

In this way, Wang et al. explored the sensitivity of NV centers as an appropriate sensor for biological imaging applications at the level of the single molecule. They used the technique as a sensor in the experimental setup to obtain the first MI of a protein at a resolution or 10 nm in situ. The scientists aim to improve the stability and sensitivity of the technique to speed up the scanning process and image a larger area of ​​interest in the cell and locate ferritin beyond the nucleus in association with additional organelles.

 Nanoscale magnetic imaging of ferritin in a single cell
(A) Ferrite cluster imaged by the NV sensor with 80 × 24 pixels and a pixel size of 8.3 nm. Scale bar, 100 nm. (B) Trace data of the scanning line in (A) directed by the red arrow. The platform indicates the ferritin cluster. The red curve fitted by a plateau serves as a guide to the eye. (C) Magnified figure of the gold dashed box in (B). The sharp transition by the red arrow around x = 283 nm shows the scanning from the blank area to the area with ferritins. Credit: Science Advances, doi: 10.1126 / sciadv.aau8038.
            

The work will contribute to clinical diagnostics to determine biomarker-based iron storage and release in cells. This will include studies on the regulatory mechanisms of iron metabolism during the progression of hemochromatosis, anemia, liver cirrhosis and Alzheimer's disease. Wang et al. propose to extend the approach in situ to other cellular components with paramagnetic signals, including magnetic molecules, metalloproteins and special spin-labeled proteins. The scientists envision further studies will explore additional targets suitable for high-resolution MI and correlated TEM imaging techniques, with optical microscopy detection incorporated in the experimental setup to extend the work and determine protein nuclear spin as well as perform three-dimensional cell tomography .
                                                                                                                        


New method discovered to view proteins inside human cells


More information:
Mamin H.J. et al. February 2013, Science. Pengfei Wang et al. Nanoscale magnetic imaging of ferritins in a single cell, Science Advances (2019). DOI: 10.1126 / sciadv.aau8038

Denis Vasyukov et al. A scanning superconducting quantum interference device with single electron spin sensitivity, Nature Nanotechnology (2013). DOI: 10.1038 / nnano.2013.169

D. Rugar et al. Single spin detection by magnetic resonance force microscopy, Nature (2004). DOI: 10.1038 / nature02658

H. J. Mamin et al. Nanoscale Nuclear Magnetic Resonance with a Nitrogen-Vacancy Spin Sensor, Science (2013). DOI: 10.1126 / science.1231540

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                                                 Nanoscale magnetic imaging of ferritin in a single cell (2019, April 18)
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