The defendants are each accused of conspiracy to commit post fraud and honest service mail fraud in which prosecutors called "Operation Varsity Blues." Authorities say the parents have paid a college prep company to cheat on standardized tests and / or bribe colleges to get their children to competitive universities.
Loughlin's husband, fashion designer Mossimo Giannulli, is also expected to come to court on Wednesday. Huffman's husband, actor William H. Macy, is not charged in the case.
CNN has reached out to representatives of Huffman, Loughlin and Giannulli, but has not heard back.
Of the 50 people charged in the case, four people have so far been guilty of guilty or planning to plead guilty, according to prosecutors.
John Vandemoer, the former Stanford sailor's head coach, has also claimed guilty of conspiracy to commit racketeering.
What the complaint says
Loughlin and Huffman are the most well-known figures in the scheme, and the accusations against them explain how the admission fraud allegedly worked.
Huffman is accused of paying $ 15,000 to a fake charity associated with Rick Singer to facilitate cheating on his daughter at SAT's, says the complaint. She discussed the scheme in a recorded phone call with Singer, the complaint says.
Dismissed, Loughlin and Giannulli obviously agreed to pay bribes totaling $ 500,000 in exchange for having their two daughters appointed as recruits to the University of Southern California crew member who facilitated their acceptance to the school.
The daughters were recruited as coxswains, even though they did not compete competitively or otherwise participated in the crew, the complaint says. The parents even sent singer pictures of each of their daughters on an ergometer, the rowing machine, the complaint says.
"I would like to thank you again for your great work with [our older daughter] she is very excited and both Lori and I are very grateful for your effort and end result!" Giannulli allegedly wrote in an email to Singer included in the complaint.
Further arrests are expected as the investigation continues, a law enforcement official acquainted with the investigation told CNN last week.
Investigators expect to charge more than five others within four to six weeks, according to the official who warned the study is ongoing, and this may change.