Grumpy Cat, the feline famous on the internet for her permanent scowl, has died aged seven, her owners say.
A statement says she died on Tuesday following complications from a recent urinary tract infection.
The cat from Arizona had "helped millions of people smile".
Grumpy, whose real name was Tardar Sauce, went viral in 201
According to owner Tabitha Bundesen, her facial expression was caused by feline dwarfism.
Grumpy Cat traveled the world making television appearances and in 2014 even starred in her own Christmas movie.
Madame Tussauds in San Francisco unveiled a waxwork of her in 2015.
Here Instagram account has more than two million followers.
In 2018, her owners won a $ 710,000 (£ 555,000) payout in a copyright lawsuit.
Grumpy Cat Limited sued the owners of the US coffee company Grenade for exceeding an agreement on the cat's image.
The company only had the right to sell its "Grumppuccino" iced drink, but was also selling other Grumpy products.
Ms Bundesen previously worked as a waitress before her beloved pet gained internet stardom. She has the express newspaper that she quit her job "within days" of Grumpy's first appearance on social media.